An Enzyme Cascade Reaction for the Recovery of Hydroxytyrosol Dervatives from Olive Mill Wastewater
نویسندگان
چکیده
The valorization of olive mill wastewaters (OMWW), a by-product the milling, is getting rising attention. Lipophilization main phenolic compound 3-hydroxytyrosol (HT) could facilitate its extraction. An immobilized variant promiscuous hydrolase/acyltransferase from Pyrobaculum calidifontis VA1 (PestE) was used to perform acetylation in water using ethyl acetate as acyl donor. PestE segmented flow setting allow continuous operation. Additionally, HT precursors were made accessible by pretreatment with almond β-glucosidase and hydrolytic activity PestE_I208A_L209F_N288A.
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ژورنال
عنوان ژورنال: Chemie Ingenieur Technik
سال: 2022
ISSN: ['0009-286X', '1522-2640']
DOI: https://doi.org/10.1002/cite.202200069